Productos de Proyectos Universitarios PAPIIT (PAPIIT_PRO)

Ing. César Núñez Hernández; L.I. Ivonne García Vázquez

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Dirección de Desarrollo Académico, Dirección General de Asuntos del Personal Académico (DGAPA)

Universidad Nacional Autónoma de México (UNAM)

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Abstract Polycyclic aromatic hydrocarbons (PAH) group represent a severe risk to health because of its carcinogenicity and mutagenicity. The microalgae Selenastrum capricornutum has potential as a biomarker and bioremediator of contamination. It has been used in experiments of exposure to different pollutants, including PAHs. Although the levels of PAHs present in contaminated water samples are close to their solubility, there are several works where concentrations higher than a hundred times, at least, are used because the methods developed don't have adequate sensitivity. This work describes the development and validation of an analytical methodology to determine the presence of four PAH: benzo[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene and benzo[a]pyrene in cultures of the green microalgae Selenastrum capricornutum. The metabolites of benzo[a]pyrene, 4,5-dihydrodiol benzo[a]pyrene, 9,10-dihydrodiol benzo[a]pyrene, 3-hydroxy benzo[a]pyrene and 9-hydroxy benzo[a]pyrene were also included. The methodology consisted of three parts: 1) separation of liquid media from biomass samples by centrifugation of pure cultures, 2) off-line extraction of analytes from biomass by a miniaturized matrix solid phase dispersion (MSPD) method and from liquid media by a solid phase extraction (SPE) method and 3) on-line SPE preconcentration and analysis of the MSPD and SPE extracts, separately, by high performance liquid chromatography with fluorescence detection (HPLC-FD). The off-line/on-line (MSPD/SPE-HPLC-FD) method was validated over a concentration range of 20 – 120 pg mg-1; good linearity (r2 > 0.9921) and precision values (RSD < 5 %) were obtained, recovery values were between 38% and 65%, and the limits of detection (LOD) were 2 – 6.5 pg mg−1. The off-line/on-line (SPE/SPE-HPLC-FD) method was validated over a concentration range of 5 – 80 pg mL-1; again good linearity (r2 > 0.9944) and precision were obtained with RSD < 7.36 %, recovery values were between 38% and 76% and the LOD were in the range of 0.8 – 2.3 pg mL−1. Samples of 15 mL of cultures exposed to two different concentrations of BaP (5 and 50 ng mL-1) were analyzed at different exposure times between 45 min and 48 h. The method developed was suitable for measuring the amount of BaP residual and metabolites produced. Assays were done by triplicate and the results showed that the metabolites favored by the metabolism of the microalgae are the dihydrodiols. Maximum amounts of these compounds between 600-6000 pg were recovered. In contrast, amounts between 60-80 pg were recovered for 9-hidoxy-BaP. In both assays, the microalgae cultures were able to decrease the BaP levels in the liquid medium below the EPA limit (<0.2 ng mL-1). Difusión: Internacional

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Con agradecimiento al PAPIIT

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2012

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Resultado directo de la investigación Proyecto PAPIIT: Desarrollo de métodos analíticos miniaturizados para la evaluación de microorganismos como bioindicadores de contaminantes orgánicos persistentes Martha Patricia García Camacho Facultad de Química https://datosabiertos.unam.mx/DGAPA:PAPIIT:IN223111 2011

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Dirección de Desarrollo Académico, Dirección General de Asuntos del Personal Académico (DGAPA), %%Extraction and analysis of benzo(a)pyrene and metabolites in microalgae cultures by off-line/on-line MSPD-SPE and HPLC-FD methods%%, objeto digital: Productos de Proyectos Universitarios PAPIIT (PAPIIT_PRO). En %%Portal de datos abiertos UNAM%% (en línea), México, Universidad Nacional Autónoma de México. Disponible en: http://datosabiertos.unam.mx/DGAPA:PAPIIT_PRO:067777 Fecha de actualización: @modified@ Fecha de consulta:

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Para más información sobre los Proyectos PAPIIT, favor de escribir a: Dra. Claudia Cristina Mendoza Rosales, directora de Desarrollo Académico (DGAPA). Correo: ccmendoza #para# dgapa.unam.mx